Table 2. Antibodies and immunohistochemical procedures.
| Antigen | Clone¤ | Manufacturer | Isotype | Incubation | Dilution | Fixation and time |
|---|---|---|---|---|---|---|
| Bcl-2 | 100 | Santa Cruz* | IgG1 | 18 h | 1:10 | Acetone, 10 min |
| CD8 | C8/144B | DAKO** | IgG1 | 30 min | 1:200 | PFA 2%, 8 min |
| CD68 | KP1 | Santa Cruz | IgG1 | 30 min | 1:400 | PFA 2%, 8 min |
| CD163 | GHI/61 | Santa Cruz | IgG1 | 30 min | 1:400 | PFA 2%, 8 min |
| FAS | B-10 | Santa Cruz | IgG1 | 18 h | 1:800 | Acetone, 10 min |
| Granzyme B | GB11 | Santa Cruz | IgG1 | 30 min | 1:1600 | PFA 2%, 8 min |
| HSP70 | C92F3A-5 | Santa Cruz | IgG1 | 30 min | 1:400 | PFA 2%, 8 min |
| Merosin 80 | MAB1922 | Millipore# | IgG1 | 30 min | 1:100 | Unfixed |
| MyHC-fast | WB-MHCf | Leica## | IgG1 | 30 min | 1:200 | Acetone, 10 min |
| MyHC-slow | WB-MHCs | Leica | IgG1 | 30 min | 1:400 | Acetone, 10 min |
| Spectrin | RBC2/3D5 | Leica | IgG2b | 30 min | 1:50 | PFA 2%, 8 min |
¤ All monoclonal antibodies were from mice and all incubations were at room temperature.
MyHC, myosin heavy chain; PFA: paraformaldehyde.
* Santa Cruz Biotechnology, Inc. Dallas TX;
** Dako Denmark A/S; # Millipore Corp, Temecula, CA; ## Leica Biosystems, Nussloch GmbH.