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. 2020 Sep 11;14:3683–3695. doi: 10.2147/DDDT.S245445

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© 2020 Zhang et al.

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Overexpression of SNHG4 reduced activation and inflammatory response of microglia via regulating miR-449c-5p. (A) qRT-PCR was employed to detect the expression of M1 markers iNOS and CD86 and M2 markers Arg1 and CD206 mRNA in microglia treated with OGD; (B) The changes of pro-inflammatory cytokines (IL-1 β, IL-6, TNF- α) and anti-inflammatory cytokines (IL-4, IL-10, TGF- β) were detected by ELISA; (C) The effect of microglia culture medium on the activity of hippocampal neurons was detected by CCK-8 assay; (D) The release of LDH from hippocampal neurons was detected. ** P< 0.01 and ***P< 0.001 versus the control group; #P< 0.05, ##P< 0.01 and ###P< 0.001 versus the NC mimics + OGD group; ^^^P< 0.001 versus the miR-449c-5p mimics + pc-NC + OGD group.

Abbreviations: iNOS, induced nitric oxide synthase; CD, cluster of differentiation; NC, negative control; pc, pc-DNA3.1; OGD, oxygen-glucose deprivation; TNF, tumor necrosis factor; IL, interleukin; NeuN, neuron; TGF, transforming growth factor; LDH, lactate dehydrogenase.