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. Author manuscript; available in PMC: 2020 Sep 16.
Published in final edited form as: Plant J. 2014 Sep 30;80(4):615–628. doi: 10.1111/tpj.12657

Figure 1.

Figure 1.

Validation of sequencing results by real-time PCR and in situ hybridization.

(a) Transcript levels of 14 genes in wild-type (WT) and spl ovules. Transcript levels were measured by qRT-PCR and normalized to HK2. Each value represents the mean of two biological replicates and two technical replicates. Error bars represent standard deviation.

(b) Whole-mount in situ hybridization of At1g15460.

(c) Whole-mount in situ hybridization of At2 g33750. Arrowheads point to the enriched expression pattern in the female meioctye (fm). Scale bars: 10 μm. Abbreviations: ii, inner integument; oi, outer integument.

(d) Heat map of the relative expression levels of six FM genes in different tissues measured by qRT-PCR. Colors are scaled by log2-transformed mean expression values from two biological replicates and two technical replicates. Black denotes high expression and white denotes low expression.