Figure S2.

SARS-CoV-2-Specific CD4⁺ T Cell Responses, Related to Figure 2

(A) Gating strategy for identification of SARS-CoV-2-antigen-specific CD4⁺ T cells.

(B) % of OX40⁺4-1BB⁺ CD4⁺ T cells specific for SARS-CoV-2 MP_R, S, M, N peptide megapools by AIM

(C) Combined % Ox40⁺4-1-BB⁺ CD4⁺ T cells across all SARS-CoV-2 peptide megapools by AIM. The black dotted line indicates LOD; the green dotted line demarcates marginal responses as determined by unexposed donor responses. Pink dots denote samples where two or more peptide pools were not run due to cell numbers.

(D-F) Amount (pg/mL) of (D) IL-5 (E), IL-13 (F), and IL-17 in the AIM supernatants after stimulation with MP_R, S, M, N, and CMV peptide pools. The black dotted line delineates background signal as determined by the unexposed controls. Acute (Ac) = Red, Convalescent (Co) = black, Unexposed (Unexp) = gray.

^∗p < 0.05, ^∗∗p < 0.01, NS = not significant.