Figure S4.
Coordinated Adaptive Immune Responses, Related to Figure 4
(A-B) PSV neutralizing titer (A) and (B) percentage of SARS-CoV-2-specific TFH cells in samples that had a positive total CD4+ AIM response (> 0.04%) following stimulation with the SARS-CoV-2 S MP, or the total CXCR5+ CD4+ T cells in unexposed controls in additional unexposed donors (n = 12) and convalescent COVID-19 donors (n = 11).
(C-D) Flow cytometry of AIM+ CD4+ T cell response (C) and AIM+ CD8+ T cells response (D) in donor C4844.
(E) Frequency of ADIM observed in the cohort.
(F) Correlation of SARS-CoV-2-specific total CD4+ T cells by OX40+CD40L+ AIM assay and days PSO, stratified into Immunotypes. Statistics are reported for unexposed, convalescent and acute samples.
(G) Frequency of ADIM by gender.
(H-I) Combined CD4+ AIM data based on day PSO from Fig. 2D and 3D with responses labeled for specific donors of interest. Statistics in (H-I) are reported for unexposed, convalescent and acute samples. The black dotted line indicates LOD; the green dotted line demarcates marginal responses as determined by unexposed donor responses. Pink dots denote samples where two or more peptide pools were not run due to cell numbers.
NS = not significant, ∗∗∗p < 0.001, geometric mean with geometric SD displayed in S4A, median displayed in S4B.