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. 2020 Sep 16;6(38):eaba8196. doi: 10.1126/sciadv.aba8196

Fig. 5. Gli1 is required for timed Tgfβ2 induction and correct temporal output.

Fig. 5

(A and B) Expression of Tgfβ2 and Phox2b in Nkx2.2 domain at r2/3 level of WT and Gli1−/− embryos at indicated stages. (C to E) Quantification of medially located Nkx2.2+ NSCs expressing Phox2b at E10.5, Isl1+MNs at E10.5, Isl1+MNs in MS at E11.5, and Lmx1b+5HTNs at E11.5 in WT and Gli1−/− embryos. Data from WT n = 3 and Gli1−/− n = 4 embryos per genotype at E10.5 and from WT and Gli1−/− n = 4 embryos per genotype at E11.5. Error bars, means ± SD. Asterisks, Student’s t test, **P ≤ 0.01 and ***P ≤ 0.001. (F) Summary of response properties of Tgfβ2 and Phox2b to GliA activity.