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. 2020 Sep 16;6(38):eabb1328. doi: 10.1126/sciadv.abb1328

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 2 — (A) Crystal structure at 3.8 Å of the BG505 UFO.664 (gp120, blue; gp41, pink) Env trimer in complex with Fabs 438-B11 (green) at the N332/V3 supersite and 35O22 (yellow) at the gp120/gp41 interface. (B) Side view of one protomer of the BG505 UFO.664 trimer in complex with Fab 438-B11 (HC, green; and LC, gray) and Fab 35O22 (yellow) Fabs. The cartoon representation of the structure is overlaid on the transparent molecular surface. The right inset shows the interaction of the 438-B11 Fab with Asn³⁰¹ and Asn³³² glycans (pink). The glycans are shown in sticks and overlaid on the transparent molecular surface. Below the inset is a schematic of Asn³⁰¹ and Asn³³² glycans observed in the structure with GlcNAc moieties as dark blue squares and mannose residues as green circles. The surface area (Å²) on each glycan moiety that is buried by 438-B11 (HC, green; LC, gray) is shown. (C) Recognition of the GDIR motif (blue) at the base of V3 by Fab 438-B11. The heavy chain (HC) 438-B11 is represented in cartoon and sticks with the CDRs and FRs (H1, green; H2, brown; H3, pink; FR1, cyan; and FR3, gray) that interact with gp120 (blue). (D) Buried surface area (Å²) of residues on the CDR loops and FRs of 438-B11 when bound to gp120 on Env. (E) Comparison of the mode of binding of N332/V3-class bNAbs to the surface of gp120 (blue) of HIV-1 Env. A distinct orientation of 438-B11 (HC, green; LC, gray) on HIV-Env is observed compared to other bNAbs of this class. HCDR3 loops (pink) are shown in cartoon representation. (F) Alignment of the amino acid sequences of HCDR3 of various N332/V3-class bNAbs.