Table 2.
Summary of pathology, viral loads, immunohistochemistry (IHC), and RNAscope in situ hybridization (ISH) in five patients who expired due to COVID-19 (LOQ = Limit of quantification)
| Pt | Specimen | Pathology | Viral loads in CoV N RNA copies/μg total RNA | Log10 viral loads genomic | Log10 viral load subgenomic |
|---|---|---|---|---|---|
| 1 | Heart | Patchy replacement fibrosis, but no acute changes or inflammation | 0 | < LOQ | < LOQ |
| Liver | Normal | 7783 | 3.89 | 3.16 | |
| Spleen | White pulp depletion and red pulp expansion | 11 | 1.04 | < LOQ | |
| Lung | Patchy pulmonary edema and acute alveolar hemorrhage; virus positive by IHC and ISH | 60,311,200 | 7.78 | < LOQ | |
| 2 | Heart | Mild lymphocytic infiltrate between myocytes and around small vessels and rare microthrombus | 10 | 1.00 | 3.52 |
| Liver | Central necrosis; virus positive by IHC | 2 | 0.30 | < LOQ | |
| Spleen | White pulp depletion and red pulp expansion and focal infarction | 7 | 0.85 | < LOQ | |
| Lung | Diffuse alveolar damage with septal lymphocytic infiltrate and type-II pneumocyte hyperplasia; virus positive by IHC and ISH | 21,407 | 4.33 | < LOQ | |
| Kidney | Acute tubular injury | 0 | < LOQ | < LOQ | |
| 3 | Heart | Mild lymphocytic infiltrate between myocytes and around small vessels | 0 | < LOQ | < LOQ |
| Liver | Periportal lymphocytic infiltrates | 0 | < LOQ | < LOQ | |
| Spleen | White pulp depletion and red pulp expansion | 1 | < LOQ | < LOQ | |
| Lung | Interstitial lymphocytic infiltrate and abundant alveolar macrophages | 10 | 1.00 | < LOQ | |
| Kidney | Acute tubular injury | 0 | < LOQ | < LOQ | |
| 4 | Heart | Mild lymphocytic infiltrate between myocytes and around small vessels | 120 | 2.08 | 3.25 |
| Liver | Unremarkable | 2 | 0.30 | < LOQ | |
| Spleen | White pulp depletion and red pulp expansion | 32 | 1.51 | < LOQ | |
| Lung | Patchy pulmonary edema and acute alveolar hemorrhage with prominent interstitial lymphocytic pneumonia with type-II pneumocyte hyperplasia and abundant alveolar macrophages; virus positive by IHC, ISH | 17,413 | 4.24 | < LOQ | |
| 5 | Heart | Mild lymphocytic infiltrate between myocytes and around small vessels | 0 | 0.00 | 2.95 |
| Liver | unremarkable | 1 | < LOQ | < LOQ | |
| Spleen | Necrotic due to infarction | 0 | < LOQ | < LOQ | |
| Lung | Diffuse alveolar damage and organizing alveolar hemorrhage; virus positive by IHC, ISH | 8435 | 3.93 | < LOQ | |
| Kidney | Tubular injury and acute inflammation and micro abscesses | 0 | < LOQ | < LOQ |