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Sequence of Real-Time PCR
| Gene | Primer sequence | Length (bp) |
|---|---|---|
| β-actin | F5ʹ-GTAAAGACCTCTATGCCAACA | 21 |
| R5ʹ- GGACTCATCGTACTCCTGCT | 20 | |
| Runx-2 | F5ʹ- ATCCAGCCACCTTCACTTACAC | 23 |
| R5ʹ- GGGACCATTGGGAACTGATAGG | 22 | |
| ALP | F5ʹ- TATGTCTGGAACCGCACTGAAC | 22 |
| R5ʹ- CACTAGCAAGAAGAAGCCTTTGG | 23 | |
| OPN | F5ʹ- CCAAGCGTGGAAACACACAGCC | 22 |
| R5ʹ- GGCTTTGGAACTCGCCTGACTG | 22 | |
| OCN | F5ʹ- GCCCTGACTGCATTCTGCCTCT | 22 |
| R5ʹ-TCACCACCTTACTGCCCTCCTG | 22 |
