Figure 3.

qPCR analysis of H. pylori mutant deficient in cagPAI genes growth in co-culture with FLA. (A) Growth Kinetics of H. pylori mutant ∆cagE with V. vermiformis (grey bars) and W. magna (black bars) or without amoebae (open bars) respectively at 37°C for up to 4 days. (B) Growth Kinetics of H. pylori mutant ∆cagM with V. vermiformis (grey bars) and W. magna (black bars) or without amoebae (open bars) respectively at 37°C for up to 4 days. Data are the mean ± SEM, n = 3 performed in triplicate. Statistical differences by Student's t-test comparing the indicated strains to the control in the absence of amoeba after 4 days (**: P < 0.001).