Table 2.
Regression and statistical parameter for the determination of AZI and LEVO using the proposed methods. Sa is standard deviation of intercept, Sb is standard deviation of slope and Sy/x is standard deviation of residuals.
| Parameters | Method I (Direct) |
Method II (D1) |
||
|---|---|---|---|---|
| AZI | LEVO | AZI | LEVO | |
| Linearity range (μg/mL) | 5.00–50.00 | 2.50–20.00 | 5.00–50.00 | 5.00–40.00 |
| LOQ (μg/mL) | 4.69 | 2.43 | 4.47 | 4.56 |
| LOD (μg/mL) | 1.55 | 0.80 | 1.47 | 1.50 |
| Intercept (a) | 1.72 × 10−2 | 9.35 × 10−3 | 5.68 × 10−3 | -2.59 × 10−3 |
| Slope (b) | 1.71 × 10−2 | 5.95 × 10−2 | 5.72 × 10−3 | 1.67 × 10−2 |
| Correlation coefficient (r) | 0.9996 | 0.9991 | 0.9995 | 0.9992 |
| Sa | 6.30 × 10−3 | 1.24 × 10−2 | 1.99 × 10−3 | 6.55 × 10−3 |
| Sb | 2.07 × 10−4 | 1.01 × 10−3 | 6.55 × 10−5 | 2.66 × 10−4 |
| Sb2 | 4.28 × 10−8 | 1.02 × 10−6 | 4.29 × 10−9 | 7.08 × 10−8 |
| Sy/x | 8.02 × 10−3 | 1.45 × 10−2 | 2.56 × 10−3 | 7.62 × 10−3 |
| F | 6828.17 | 3470.70 | 7624.17 | 3951.43 |
| Significance F | 3.91 × 10−6 | 1.08 × 10−5 | 1.03 × 10−7 | 8.87 × 10−6 |