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. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Aug 14;531(4):497–502. doi: 10.1016/j.bbrc.2020.07.060

Figure 2. TAOK3 regulates late-stage osteoblast differentiation through JNK/MAPK pathway.

Figure 2.

Primary COBs were isolated from Taok3+/+ and Taok3−/− mice and cultured under differentiation conditions. To address the upstream regulator of TAOK3, COBs were infected with vector or cre-recombinase expressing lentivirus followed by serial dosage of indicated stimuli.

(A) COB lysates were immunoblotted with indicated antibodies on either day 7 or day 14. Quantification of phospho-ERK and phospho-JNK levels were performed using ImageJ. ***p<0.001, **** p<0.001.

(B) COBs were cultured with 0, 10, 30, 60 micro mole (μM) As2O3 under differentiation conditions and cell lysates were immunoblotted with indicated antibodies followed by quantification.

(C) COBs were cultured with 0, 5, 15, 30 Joule per square meter (J/m2) UV under differentiation conditions and cell lysates were immunoblotted with indicated antibodies followed by quantification.