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. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Aug 14;531(4):497–502. doi: 10.1016/j.bbrc.2020.07.060

Figure 3. TAOK3 is required for differentiation of osteoblasts from human MSCs in vitro.

Figure 3.

(A) hMSCs were cultured under undifferentiated or osteogenic differentiation conditions. hMSCs were infected with a vector control or human TAOK3 shRNA bearing lentivirus to silence TAOK3 gene in vitro. TAOK3 mRNA levels were measured by qPCR analysis (n=3 per group). **** p<0.001.

(B) Von Kossa staining of hMSCs infected with vector or shRNA bearing lentivirus was performed 21 days after osteogenic differentiation.

(C) aPCR analysis was performed to measure mRNA levels of IBSP and ALPL in hMSCs 7 days after osteogenic differentiation (n=3 per group). ALP assay was performed to quantify ALP activity (n=3 per group). ** p<0.01, ***p<0.001.