(a) Concentration process of 8 μm fluorescent polystyrene particles within a single unit of the acoustofluidic multi-well plate when the driving voltage, frequency, droplet volume, and particle concentration are 6 Vpp, 112 kHz, 15 μL, and 0.10 mg/mL, respectively. Scale bar: 500 μm. (b) Concentration process of 15 μm fluorescent polystyrene particles within a single unit of the acoustofluidic multi-well plate when the driving voltage, frequency, droplet volume, and particle concentration are 6 Vpp, 112kHz, 15 μL, and 0.20 mg/mL, respectively. Scale bar: 500 μm. (c) Fluorescence intensity versus acoustic field time under different driving voltages for 15 μm fluorescent polystyrene particles when the droplet volume and particle concentration are 15 μL and 0.20 mg/mL, respectively, (d) Fluorescence intensity versus acoustic field time under different droplet volumes for 15 μm fluorescent polystyrene particles when the driving voltage and particle concentration are 6 Vpp and 0.20 mg/mL, respectively, (e) Relationship between the stable fluorescence intensity after acoustic field and particle concentration for 15 μm fluorescent polystyrene particles when the diving voltage and droplet volume are maintained at 6 Vpp and 15 μL, respectively.