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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Arch Biochem Biophys. 2020 Aug 18;692:108546. doi: 10.1016/j.abb.2020.108546

Figure 4. EGCG induces S/G2 cell cycle arrest in pancreatic cancer cells.

Figure 4.

A: Panc-1 and MIA PaCa-2 cells were treated with EGCG for 24 h, as indicated, and the cell cycle phase transition was determined by flow cytometry following PI staining. B: EGCG modulated S/G2 phases arrest protein expression. Immunoblots for phosphorylated Chk1 (p-Chk1), phosphorylated p53 (p-p53), p53, p21, cdc2, and cyclin B1 in total cell protein extracts from Panc-1 and MIA PaCa-2 cells treated with escalating concentrations of EGCG, as indicated, for 24 h. Loading control: β-Actin. Bands were quantified and results are expressed as percentage of control. *p<0.05, **p<0.01 vs. control.