Figure 1. Isoflurane, but not sevoflurane, is toxic to male and female ND23⁶⁰¹¹⁴ flies in an age-dependent manner.

(A) wild-type and (B) ND23⁶⁰¹¹⁴ male and female flies at different ages were either not exposed (Control) or exposed to 4%h isoflurane or 7%h sevoflurane in room air (21% O₂) at either 2–4 days old (2–4 d) or 10–13 days old (10–13 d) and the percent mortality was determined after 24 h. N=number of biological replicates. Symbols indicate the following: box (Q2-Q3), + (mean), horizontal bar (median), and whiskers (minimum and maximum). Significance between control and experimental data was determined using the unpaired equal-variance two-tail Student’s t-test. ****, P<0.0001. Note the difference in y-axis scale between (A) and (B).