Figure 4. Isoflurane toxicity in male and female ND23⁶⁰¹¹⁴ flies is oxygen-dependent.

Male and female wild-type (A and C) and ND23⁶⁰¹¹⁴ (B and D) flies were either not exposed (Control) or exposed to 2h of 2% isoflurane or 2h of 3.5% sevoflurane at either 2–4 days old (2–4 d) or 10–13 days old (10–13 d), and the percent mortality was determined after 24 h. Flies were exposed to 75% (A and B) or 5% (C and D) O₂ for 2 h, concurrent with the anesthetic. Note the difference in y-axis scale between (A and C) and (B and D). Also, note almost 100% mortality in panel B for isoflurane in old animals. N=number of biological replicates. Symbols indicate the following: box (Q2-Q3), + (mean), horizontal bar (median), and whiskers (minimum and maximum). Significance between control and experimental data was determined using the unpaired equal-variance two-tail Student’s t-test. *, P<0.05.