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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Anesthesiology. 2020 Oct 1;133(4):839–851. doi: 10.1097/ALN.0000000000003486

Figure 7. Mortality after exposure to isoflurane increases with age in heterozygous ND2360114 flies.

Figure 7.

ND2360114/wild-type flies of the indicated ages were exposed to 2h of 2% isoflurane in 75% O2, and the percent mortality at 24 h was determined. Each experiment consisted of four biological replicates. Symbols indicate the following: box (Q2-Q3), + (mean), horizontal bar (median), and whiskers (minimum and maximum). Significance was determined using an ordinary one-way ANOVA followed by the Dunnett post-hoc test for multiple comparisons. **, P<0.01; ****, P<0.0001.