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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Dev Biol. 2020 Aug 13;466(1-2):36–46. doi: 10.1016/j.ydbio.2020.08.004

Fig 6. Pdgfra activity promotes chondrocyte progenitor formation and proliferation.

Fig 6.

(A) Procedure of isolating and culturing eMSCs from distinct lineages of E13.5 embryonic heads. (B) Statistical analysis of CFU assay results of primary cells sorted from distinct lineages of E13.5 embryonic craniofacial cells. N=5. (C) Comparison of percentile of multipotent colonies from mesoderm and neural crest cells. (D) Q-PCR result of Sox9 and Col2a1 expression in representative eMSC colony isolated from mesoderm cells. N=3. (E) Statistical analysis of BrdU labeling assay in EGFP+ cells isolated from E12.5 Sox9+/IRES-EGFP embryos. P2 cells are treated with 10ng/ml Pdgf-aa for 12hr or 24hr. Percentile of BrdU+ cells are quantified and compared to untreated cells. N=7.