Table 1:
Validation of RNA-SEQ Hits using qRT-PCR
| Gene ID | Gene Name | Fold Regulation | P Value |
|---|---|---|---|
| Rem2 | Rad and gem related GTP binding protein 2 | −1.6149 | 0.064004 |
| Gb5 | Guanine nucleotide binding protein (G protein), beta 5 | −1.5228 | 0.012241 |
| Ptpn5 | Protein tyrosine phosphatase, non-receptor type 5 | −1.2928 | 0.437776 |
| Vgf | VGF nerve growth factor inducible | −2.4344 | 0.070605 |
| Bdnf | Brain derived neurotrophic factor | −1.3205 | 0.000487 |
| Crh | Corticotropin releasing hormone | −2.3208 | 0.036148 |
| Grk1 | G protein-coupled receptor kinase 1 | −1.121 | 0.597184 |
| Gcap1 | Guanylate cyclase activator 1a (retina) | −1.127 | 0.198999 |
| Aipl1 | Aryl hydrocarbon receptor-interacting protein-like 1 | −1.1622 | 0.175192 |
| BC027072 | CDNA sequence BC027072 | −1.1869 | 0.338464 |
| Notch1 | Notch gene homolog 1 (Drosophila) | −1.2042 | 0.375271 |
| Sox9 | SRY-box containing gene 9 | 1.3214 | 0.026125 |
| Syt7 | Synaptotagmin VII | 1.0844 | 0.469485 |
| Hrh3 | Histamine receptor H3 | 1.0346 | 0.793852 |
| Nlgn1 | Neuroligin 1 | 1.2214 | 0.16884 |
| Cnr1 | Cannabinoid receptor 1 (brain) | 1.2974 | 0.163387 |
| Enpp2 | Ectonucleotide pyrophosphatase/phosphodiesterase 2 | 1.0903 | 0.780956 |
| Serpine2 | Serine (or cysteine) peptidase inhibitor, clade E, member 2 | 1.1261 | 0.605115 |
| Nlgn3 | Neuroligin 3 | −1.1403 | 0.484625 |
| Calcrl | Calcitonin receptor-like | 1.0237 | 0.749487 |
| Slc25a13 | Solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13 | 1.0944 | 0.646481 |
| Cplx3 | Complexin 3 | 12.2884 | 0.039817 |
| Slc13a3 | Solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3 | 1.1296 | 0.848099 |
| Arl6ip1 | ADP-ribosylation factor-like 6 interacting protein 1 | −1.0689 | 0.439723 |
RNA for this study was identical to those samples employed for RNA-Seq studies. The p values are calculated based on a Studenťs t-test of the replicate 2^(- Delta CT) values for each gene in the control group and treatment groups, and p values less than 0.05 are indicated in red. These data reflect relative quantification whereas the RNA-seq data is absolute quantification.