Fig. 2.

Cytokine release from PBMC from patients with ACS and controls primed with combination of C5a and TNF and stimulated with CC. PBMC were isolated from blood from patients with ACS and healthy controls, primed with C5a+TNF or left unprimed prior to incubation with CC and supernatant collected. IL-1β and IL-1α released is presented for the two groups as mean ± SEM, n=9-10. Basal levels and control treatments are presented in Supplementary figure I. ACS, acute coronary syndrome; C, Complement factor; CC, cholesterol crystals; IL, interleukin; TNF, tumour necrosis factor. **p<0.01 between controls and ACS with priming, Mann-Whitney two-tail test.