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. 2020 Sep 10;60:102989. doi: 10.1016/j.ebiom.2020.102989

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 3 — In vitro loss and gain of PDPN function in MSC and NSC.

(a) PDPN expression in sMSC was suppressed by siRNA (siPDPN). Shown by Western Blot. Migration was analysed for untransfected (w/o) sMSC, sMSC incubated with transfection medium (TM) only, siPDPN transfected sMSC, and non-transfected (w/o) oMSC (migration time: 3 h), n=5, each data point represents one biological replicate per group out of a single experiment; ANOVA and Bonferroni's multiple comparisons test was used to compare non-transfected (w/o) oMSC with siPDPN transfected or untransfected sMSC, (***p <0.001). Error bars: SEM.

(b) Migration over 4 h of untransfected (w/o) oMSC/oNSC, oMSC/oNSC incubated with TM only, and PDPN overexpressing oMSC/oNSC was analysed after 120 h (oMSC) or 72 h (oNSC) after PDPN transfection, n=6 (oMSC), n=5 (oNSC), each data point represents one biological replicate per group; ANOVA and Bonferroni's multiple comparisons test was used to compare PDPN transfected cells with TM-treated or untransfected (w/o) oMSC/oNSC (*p<0.05; ***p<0.001). Error bars: SEM.