Fig. 7.

Aβ pathology and memory deficits after intranasal oMSC or sMSC treatment in transgenic AD mice

(a) Human Aβ 40 and 42 quantified by ELISA in 7-month-old APP23/PS45 mice (n=4 per group), 6-month-old 3xTg-AD mice (n=5 per group) and 13-months-old 3xTg-AD mice (n=5 per group) after INA of o/sMSC (2x application of 1 × 10⁶ cells). Aβ plaque number and cumulative area in the hippocampi of 13-month-old 3xTg-AD mice (n=3 per group) after INA of o/sMSC (2x application of 1 × 10⁶ cells). Number of plaques and cumulative area per brain section quantified from 9-10 brain sections per animal using the APP/Aβ antibody 6E10, each data point represents plaque number or plaque area calculated per section. Each column represents 8-10 data points obtained from 9-10 sections. To compare vehicle-, oMSC and sMSC-treated groups ANOVA with Bonferroni's multiple comparisons test was used (*p <0.05; **p <0.01). Error bars: SEM.

(b) Performance in T-maze of 6- and 13-month-old 3xTg-AD mice (n=8 per group) prior (test block 1) and after (test blocks 2-4) INA of o/s MSC (2x application of 1 × 10⁶ cells) shown in test blocks with 4 consecutive test days in each block. Experiments performed once with 6-month-old mice, and twice in 13-month-old mice. The test blocks 2-4 were performed from day 13 to day 28 after INA. In the “correct choice” assessment, each bar represents 32 values of correct choice percentages out of 6 runs on 4 consecutive days of one test block assessed from 8 animals per group. In the latency assessment each bar represents 24-42 data points of latency to reach the correct arm on the last day of each test block from 6-8 mice out of 8 mice analysed. #-comparison between oMSC and sMSC, *-comparison between o/sMSC and PBS (vehicle) group, §-comparison of the respective treatment group in test blocks after INA vs. test block 1 (for instance oMSC of test block 1 vs. oMSC in test blocks 2-4). ANOVA with Bonferroni's multiple comparisons test was used (*p <0.05; **p <0.01; ***p<0.001). Error bars: SEM.