. 2020 Apr 14;36(4):407–413. doi: 10.1007/s43188-020-00047-0

Table 1.

Composition of assay mixtures (ml) for Gloydius snake antivenom titer determination

	Preparation	(1)	(2)	(3)	(4)	(5)
Antivenom	200 U/ml (anti-lethal titer) or 20 U/ml (anti-hemorrhagic titer)	0.320	0.400	0.500	0.630	0.800
GPBS^a		0.680	0.600	0.500	0.370	0.200
Venom	10 test dose/ml	1.000	1.000	1.000	1.000	1.000

Preparation

(1)

(2)

(3)

(4)

(5)

Antivenom

200 U/ml (anti-lethal titer) or

20 U/ml (anti-hemorrhagic titer)

0.320

0.400

0.500

0.630

0.800

GPBS^a

0.680

0.600

0.500

0.370

0.200

Venom

10 test dose/ml

1.000

^a0.017 mol/l phosphate buffered sodium chloride solution containing 0.2 w/v% gelatin (pH 7.0)