Table 3.
The results of the micronucleus test, means [95%, CI] (‰)
| Cultivation time | The frequency of micronucleus test’s indicators (‰) | ||||
|---|---|---|---|---|---|
| 0 µg/ml NaF | 20 µg/ml NaF | 100 µg/ml NaF | 200 µg/ml NaF | 12.5 ng/ml Mitomycin C | |
| MNBN | |||||
| 48 h | 32.33 [25.16–9.50] | 42.00 [24.90–9.09] | 60.67 [51.26–70.07]* | 69.11 [62.08–76.13]** | 88.78 [82.57–94.98] |
| 72 h | 35.00 [17.09–2.91] | 32.00 [25.42–8.57] | 73,91 [61.34–86.48]** | 90.21 [88.82–91.60]** | 100.07 [76.42–123.71] |
| Binucleated cells with nucleoplasmic bridges | |||||
| 48 h | 7.67 [0–15.41] | 9.33 [1.74–16.92] | 23.66 [19.87–27.46]** | 21.82 [19.22–24.42]* | 33.98 [28.05–39.90] |
| 72 h | 7.66 [1.93–13.40] | 12.67 [6.63–18.71] | 17.32 [9.52–25.13]* | 24.54 [20.44–28.64]** | 38.09 [29.29–46.89] |
| Binucleated cells with nuclear protrusion | |||||
| 48 h | 11.67 [7.87–15.46] | 6.67 [0–13.84] | 18.67 [7.46–29.87] | 33.43 [25.03–41.83]* | 27.02 [19.5–34.54] |
| 72 h | 7.33 [5.90–8.77] | 7.67 [4.80–10.53] | 22.73 [18.61–26.85]* | 23.41 [21.16–25.67]* | 29.19 [22.36–36.01] |
*p < 0.05, Significant differences against samples treated with 0 µg/ml of sodium fluoride; **p < 0.01, Significant differences against samples treated with 0 µg/ml of sodium fluoride