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. 2020 Apr 14;36(4):367–406. doi: 10.1007/s43188-020-00042-5

Table 8.

Thirteen-weeks repeated dose toxicity and four-weeks recovery study: hematological values of male rats (main study) and hematological values of female rats (mains study)

Summary of hematological tests (sex: male)
Test item Main group (mg/kg/day)
G1 (0) G2 (500) G3 (1000) G4 (2000)
WBC1 (K/μL) 6.66 ± 1.46 (10) 8.57 ± 1.57 (10) 8.53 ± 3.35 (10) 9.33 ± 2.89 (10)
NE2 (K/μL) 0.96 ± 0.28 (10) 1.25 ± 0.55 (10) 1.22 ± 0.48 (10) 1.33 ± 0.53 (10)
EO3 (K/μL) 0.06 ± 0.03 (10) 0.10 ± 0.07 (10) 0.09 ± 0.04 (10) 0.08 ± 0.04 (10)
BA4 (K/μL) 0.01 ± 0.01 (10) 0.00 ± 0.01 (10) 0.01 ± 0.01 (10) 0.01 ± 0.01 (10)
LY5 (K/μL) 5.37 ± 1.29 (10) 6.95 ± 1.47 (10) 6.91 ± 2.93 (10) 7.62 ± 2.30 (10)
MO6 (K/μL) 0.14 ± 0.07 (10) 0.18 ± 0.06 (10) 0.17 ± 0.08 (10) 0.17 ± 0.08 (10)
LUC7 (K/μL) 0.12 ± 0.08 (10) 0.10 ± 0.03 (10) 0.12 ± 0.08 (10) 0.13 ± 0.07 (10)
NEP8 (%) 14.8 ± 3.9 (10) 14.8 ± 6.1 (10) 15.0 ± 5.2 (10) 14.0 ± 3.0 (10)
EOP9 (%) 1.0 ± 0.5 (10) 1.2 ± 0.8 (10) 1.1 ± 0.4 (10) 0.8 ± 0.3 (10)
BAP10 (%) 0.1 ± 0.1 (10) 0.1 ± 0.1 (10) 0.1 ± 0.1 (10) 0.0 ± 0.0 (10)
LYP11 (%) 80.4 ± 5.0 (10) 80.8 ± 7.2 (10) 80.5 ± 5 2 (10) 82.1 ± 3.7 (10)
MOP12 (%) 2.1 ± 0.9 (10) 2.1 ± 0.7 (10) 1.9 ± 0.6 (10) 1.7 ± 0.5 (10)
LUP13 (%) 1.8 ± 0.9 (10) 1.1 ± 0.2 (10) 1.4 ± 0.4 (10) 1.3 ± 0.4 (10)
RBC14 (M/μL) 7.94 ± 0.28 (10) 7.92 ± 0.35 (10) 7.68 ± 0.55 (10) 7.93 ± 0.34 (10)
Hb15 (g/dL) 14.3 ± 0.6 (10) 14.6 ± 0.9 (10) 14.4 ± 0.4 (10) 14.4 ± 0.5 (10)
RDW16 (%) 12.8 ± 1.0 (10) 12.7 ± 0.8 (10) 12.7 ± 0.8 (10) 12.5 ± 0.9 (10)
HCT17 (%) 44.3 ± 1.9 (10) 44.8 ± 2.7 (10) 43.8 ± 2.4 (10) 44.7 ± 1.4 (10)
MCV18 (fL) 55.8 ± 1.5 (10) 56.5 ± 2.1 (10) 57.1 ± 2.7 (10) 56.4 ± 1.4 (10)
MCH19 (pg) 18.0 ± 0.5 (10) 18.4 ± 0.8 (10) 18.8 ± 1.2 (10) 18.2 ± 0.4 (10)
MCHC20 (g/dL) 32.4 ± 0.5 (10) 32.6 ± 1.0 (10) 32.9 ± 1.4 (10) 32.3 ± 0.5 (10)
Retic21 (%) 2.21 ± 0.45 (10) 2.36 ± 0.37 (10) 2.42 ± 0.74 (10) 2.13 ± 0.53 (10)
PLT22 (K/μL) 901 ± 86 (10) 892 ± 150 (10) 927 ± 121 (10) 957 ± 150 (10)
MPV23 (fL) 5.0 ± 0.2 (10) 4.9 ± 0.2 (10) 5.0 ± 0.3 (10) 5.1 ± 0.2 (10)
Summary of hematological tests (sex: female)
Test item Group (mg/kg/day)
G1 (0) G2 (500) G3 (1000) G4 (2000)
WBC1 (K/μL) 3.99 ± 0.49 (10) 4.32 ± 1.15 (10) 5.04 ± 1.33 (10) 5.36 ± 1.06* (10)
NE2 (K/μL) 0.74 ± 0.15 (10) 0.87 ± 0.60 (10) 0.67 ± 0.21 (10) 0.95 ± 0.34 (10)
EO3 (K/μL) 0.06 ± 0.01 (10) 0.06 ± 0.03 (10) 0.07 ± 0.03 (10) 0.08 ± 0.02 (10)
BA4 (K/μL) 0.01 ± 0.01 (10) 0.01 ± 0.01 (10) 0.01 ± 0.01 (10) 0.00 ± 0.01 (10)
LY5 (K/μL) 3.08 ± 0.57 (10) 3.24 ± 0.85 (10) 4.13 ± 1.23* (10) 4.17 ± 0.82* (10)
MO6 (K/μL) 0.10 ± 0.04 (10) 0.12 ± 0.05 (10) 0.14 ± 0.06 (10) 0.14 ± 0.07 (10)
LUC7 (K/μL) 0.02 ± 0.01 (10) 0.02 ± 0.01 (10) 0.02 ± 0.02 (10) 0.02 ± 0.01 (10)
NEP8 (%) 18.9 ± 4.8 (10) 19.6 ± 8.8 (10) 13.8 ± 4.9 (10) 17.6 ± 4.8 (10)
EOP9 (%) 1.4 ± 0.3 (10) 1.5 ± 0.4 (10) 1.5 ± 0.5 (10) 1.4 ± 0.4 (10)
BAP10 (%) 0.1 ± 0.1 (10) 0.2 ± 0.1 (10) 0.2 ± 0.2 (10) 0.1 ± 0.1 (10)
LYP11 (%) 76.7 ± 5.5 (10) 75.7 ± 9.0 (10) 81.4 ± 5.7 (10) 78.0 ± 5.3 (10)
MOP12 (%) 2.4 ± 0.9 (10) 2.6 ± 0.7 (10) 2.7 ± 1.2 (10) 2.6 ± 1.2 (10)
LUP13 (%) 0.4 ± 0.1 (10) 0.5 ± 0.3 (10) 0.4 ± 0.2 (10) 0.3 ± 0.2 (10)
RBC14 (M/μL) 7.70 ± 0.24 (10) 7.58 ± 0.31 (10) 7.61 ± 0.27 (10) 7.41 ± 0.42 (10)
Hb15 (g/dL) 14.5 ± 0.4 (10) 14.6 ± 0.5 (10) 14.6 ± 0.3 (10) 14.4 ± 0.7 (10)
RDW16 (%) 11.0 ± 0.2 (10) 11.1 ± 0.3 (10) 10.9 ± 0.3 (10) 10.9 ± 0.5 (10)
HCT17 (%) 43.6 ± 1.5 (10) 43.2 ± 1.4 (10) 43.3 ± 1.3 (10) 43.0 ± 2.1 (10)
MCV18 (fL) 56.6 ± 1.0 (10) 57.0 ± 1.0 (10) 56.8 ± 1.3 (10) 58.1 ± 1.3* (10)
MCH19 (pg) 18.9 ± 0.6 (10) 19.3 ± 0.5 (10) 19.2 ± 0.5 (10) 19.4 ± 0.5 (10)
MCHC20 (g/dL) 33.6 ± 0.6 (10) 33.9 ± 0.6 (10) 33.7 ± 0.7 (10) 33.5 ± 0.6 (10)
Retic21 (%) 1.78 ± 0.35 (10) 1.95 ± 0.30 (10) 1.89 ± 0.42 (10) 1.98 ± 0.50 (10)
PLT22 (K/μL) 1048 ± 131 (10) 1007 ± 72 (10) 1076 ± 130 (10) 1034 ± 74 (10)
MPV23 (fL) 5.1 ± 0.2 (10) 5.0 ± 0.2 (10) 5.1 ± 0.3 (10) 4.9 ± 0.2 (10)

1: white blood cell, 2: neutrophil, 3: eosinophil, 4: basophil, 5: lymphocyte, 6: monocyte, 7: large unstained cell, 8: percent of neutrophil, 9: percent of eosinophil, 10: percent of basophil, 11: percent of lymphocyte, 12: percent of monocyte, 13: percent of large unstained cell, 14: red blood cell, 15: hemoglobin, 16: red cell distribution width, 17: hematocrit, 18: mean corpuscular volume, 19: mean corpuscular hemoglobin, 20: mean corpuscular hemoglobin concentration, 21: reticulocyte, 22: platelet, 23: mean platelet volume

Mean ± SD (Number of animals)

Significant difference compared with the control group value: *p < 0.05