Table 3.
Primer sequences of the targets used in nanoscale chip PCR.
| Target | Primer Pair | Forward | Reverse |
|---|---|---|---|
| B7-1 | 1 | GCTGTCCTGTGGTCACAATG | TGCCAGTAGATGCGAGTTTG |
| 2 | CACCTCTCCTGGTTGGAAAA | TAAGGTAATGGCCCAGGATG | |
| B7-2 | 1 | GTATTTTGGCAGGACCAGGA | CTTGTGCGGCCCATATACTT |
| 2 | GTATTTTGGCAGGACCAGGA | CGGCCCATATACTTGGAATG | |
| PD-L1 | 1 | TATGGTGGTGCCGACTACAA | TGACTGGATCCACAACCAAA |
| 2 | CGAAGTCATCTGGACAAGCA | CTCTTGGAATTGGTGGTGGT | |
| PD-L2 | 1 | GGAACTTACTTTGGCCAGCA | GATGCAGAAGGGGATGAAAA |
| 2 | AAGTCCAAGTGAGGGACGAA | GGCGACCCCATAGATGATTA | |
| B7-H2 | 1 | GTCCTGGACTGCTCTTCCTG | CCATCGCTCTGACTTCCTTC |
| 2 | GTCCTGGACTGCTCTTCCTG | TCGCTCTGACTTCCTTCTCC | |
| B7-H3 | 1 | GTGGGGCTGTCTGTCTGTCT | TGATCTTTCTCCAGCACACG |
| 2 | GTCTCATTGCACTGCTGGTG | TGATCTTTCTCCAGCACACG | |
| B7-H4 | 1 | GTCGGAGCAGGATGAAATGT | TGAGTTGCACGTTTTTCAGC |
| 2 | GCTGAAAAACGTGCAACTCA | TAGCATTCCCCTTGCCTTTA | |
| B7-H5 | 1 | GGCAACTTCTCCATCACCAT | CAGTAGAGGCCGCTATCCAG |
| 2 | GCATCGTAGGAATCCTCTGC | CCTGCCTTTGCTTGTAGACC | |
| B7-H6 | 1 | CACACCCCTGAATGACAATG | TGTTGAGGGGTTGGGAATAA |
| 2 | CTGAAGGCACAGGGAACAGT | TTGATCCAGCAACAATCTGC | |
| B7-H7 | 1 | GACCATTTGGAAAGCCAAGA | CGCATTCCCATTTTGAATCT |
| 2 | TAGAAGCCAGGAGGAGCAGA | CCAGGAGGGACACAACATCT | |
| IFNγ | 1 | TTCAGCTCTGCATCGTTTTG | ATGGGTCCTGGCAGTAACAG |
| 2 | TCATCCAAGTGATGGCTGAA | CTTCGACCTCGAAACAGCAT | |
| TNFα | 1 | AACCTCCTCTCTGCCATCAA | GGAAGACCCCTCCCAGATAG |
| 2 | GAGAAGGGTGACCGACTCAG | CCAAAGTAGACCTGCCCAGA | |
| TGFβ | 1 | GTACCTGAACCCGTGTTGCT | CACGTGCTGCTCCACTTTTA |
| 2 | AGCTCCACGGAGAAGAACTG | GTCCTTGCGGAAGTCAATGT | |
| IL-10 | 1 | GAGAACAGCTGCACCCACTT | TCTCGGAGATCTCGAAGCAT |
| 2 | TTACCTGGAGGAGGTGATGC | GCCTTGATGTCTGGGTCTTG | |
| IL-2 | 1 | TCACCAGGATGCTCACATTT | GCACTTCCTCCAGAGGTTTG |
| 2 | CCCAGGGACTTAATCAGCAA | ATGGTTGCTGTCTCATCAGC | |
| IDO | 1 | GCCCTTCAAGTGTTTCACCAA | CCAGCCAGACAAATATATGCGA |
| 2 | CCTGAGGAGCTACCATCTGC | GCTTGCAGGAATCAGGATGT | |
| TDO | 1 | CAAATCCTCTGGGAGTTGGA | GTGCATCCGAGAAACAACCT |
| 2 | GACGGCTGTCATACAGAGCA | CCTGGAACCTAGGCTCTTCC | |
| Perforin | 1 | ACTCACAGGCAGCCAACTTT | GGGTGCCGTAGTTGGAGATA |
| 2 | CCCTCTGTGAAAATGCCCTA | GGCTTAGGAGTCACGTCCAG | |
| Granzyme B | 1 | ACTGCAGCTGGAGAGAAAGG | CTGGGCCTTGTTGCTAGGTA |
| 2 | GGAGGCCCTCTTGTGTGTAA | TGCCATTGTTTCGTCCATAG | |
| PD-1 | 1 | TGCAGCTTCTCCAACACATC | CATGCGGTACCAGTTTAGCA |
| 2 | GTGCCTGTGTTCTCTGTGGA | TTCTCTCGCCACTGGAAATC | |
| Osteopontin | 1 | CATCACCTGTGCCATACCAG | GCCACAGCATCTGGGTATTT |
| 2 | TGAAACGAGTCAGCTGGATG | GCTCTCATCATTGGCTTTCC | |
| Glypican-3 | 1 | CCAACATGCTGCTCAAGAAA | TCCATGTTCAATCGTGCTGT |
| 2 | CTGGATGAGGAAGGGTTTGA | AGCCTCCAATGCACTCATCT |