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. 2020 Sep 3;8:914. doi: 10.3389/fbioe.2020.00914

Table 1.

List of microalgae and diatoms with complete or near to complete genome, and the overview of reported omics studies.

Organism (strain used for genome sequencing) Genome size (Mb) Conditions or aim of omics studies Focus Accession numbers and references
Transcriptomic studies Proteomic studies Metabolomic studies/ metabolic models
Auxenochlorella protothecoides
(0710)
22.92 Response to temperature and phosphate stress; trophic growth conditions; oil accumulation Response to temperature, nitrogen and phosphorus starvation, ionizing radiation; trophic growth conditions, oil accumulation, Response to temperature and, phosphate and nitrogen starvation, copper stress; oil accumulation, glycome profiling, trophic growth conditions / Genome scale and core metabolic model Biofuel PRJNA428835, PRJNA484804 (Li et al., 2013, 2014b; Gao et al., 2014a; Sibi et al., 2014; Wu et al., 2015; Park and Choi, 2018; Park et al., 2018; Vogler et al., 2018; Xing et al., 2018)
Bathycoccus prasinos
(RCC 1105)
15.07 Normal growth conditions - - Comparative analysis PRJNA231566, https://www.imicrobe.us/#/projects/104
Bigelowiella natans
(CCMP2755)
91.41 High light stress and small RNA profiling Profiling of proteins targeted to plastid and peri-plastid space - Model Organism GSE124831, GSE115762 (Hopkins et al., 2012)
Botryococcus braunii
(Showa)
184.32 Response to nitrogen deprivation, high salt, cobalt enrichment, NaHCO3, salicylic acid, methyl jasmonate, and acetic acid - Response to different nutrients, growth phases; tetraterpenoid and hydrocarbons analysis / Genome scale metabolic model Hydrocarbons and biofuels FY358876, GES71296, SRP161189, GSE96585 (Molnar et al., 2012; Cornejo-Corona et al., 2016; Thapa et al., 2016; Blifernez-Klassen et al., 2018)
Chlamydomonas debaryana
(NIES-2212)
120.36 - - Oxylipin analysis, lipid profiling in response to different light and CO2 levels de los Reyes et al., 2014; Toyoshima and Sato, 2015, 2018; Yoshitomi et al., 2019
Chlamydomonas reinhardtii
(CC-503 cw92 mt+)
120.4 Response to nutrient starvation, oxidative and heat stress, high light intensity, diurnal cycle; ciliogenesis; lipid accumulation Response to nitrogen and sulfur starvation; exposure to high salinity, high CO2, dark and anoxic conditions; lipid mutant, lipid droplet proteins Response to nitrogen starvation, dark and anoxic conditions / Genome scale and core metabolic model Model organism GSE17970, PRJNA379963 (May et al., 2009; Chen et al., 2010; Baba et al., 2011; Nguyen et al., 2011; Longworth et al., 2012; Mastrobuoni et al., 2012; Choi et al., 2013; Chaiboonchoe et al., 2014; Wase et al., 2014; Sithtisarn et al., 2017; Salguero et al., 2019)
Chlorella pyrenoidosa
(FACHB-9)
56.99 Response to CO2 deprivation, bisphenol A, salt stress, high light stress, glucose starvation and hydroxyl radical; trophic growth conditions Dried biomass, exposure to inhibitor of mitochondrial respiratory electron transport Lipid profiling under copper stress and different nitrate levels / Core metabolic model Biofuels SRX399080, GSE40028, GSE69816, PRJNA292642, PRJNA526277 (Yang et al., 2000; Sibi et al., 2014; Liu et al., 2018b; Wan et al., 2018; Zhang et al., 2018; Duan et al., 2019)
Chlorella sorokiniana
(1230)
58.53 Response to nitrogen deprivation, different pH, and high CO2 Response to inoculum sizes, light intensity and glucose concentrations, nitrogen starvation; bioactive peptide analysis Response to high-density cultivation and UV radiation; fatty acid profiling Biofuels GAPD00000000, GSE98781, GCUV00000000 (Lu et al., 2013; Ma et al., 2013; Rosenberg et al., 2014; Li et al., 2015a; Chen et al., 2017; Kumar et al., 2018; Tejano et al., 2019)
Chlorella variabilis (NC64A) 46.16 Response to early phase of Chlorella virus-1 infection - Nitrogen deprivation and long-chain alkenes/Genome scale metabolic model Biofuels SRP026413 (Juneja et al., 2016; Sorigue et al., 2016)
Chlorella vulgaris
(NJ-7)
39.08 Response to nitrogen starvation and salt stress Response to nitrogen depletion and repletion, heterotrophic and Na induced lipid accumulation, S-nitrosylated proteome in nitrogen deplete and replete condition Lipid profiling under copper stress, effect of graphene oxide nanomaterial, N-glycan profiling / Core metabolic model Biofuels LDKB00000000 (Guarnieri et al., 2011, 2013; Sibi et al., 2014; Li et al., 2015b; Ouyang et al., 2015; Henard et al., 2017; Zuñiga et al., 2018; Mocsai et al., 2019)
Chloroidium sp.
(CF)
54.31 - Normal growth conditions / Genome scale metabolic model Ecological importance Nelson et al., 2017, 2019
Chromochloris zofingiensis
(SAG 211-14)
58 Response to nitrogen deprivation, high light; heterotrophic conditions, different growth conditions Lipid droplets analysis Lipid and carotenoid profiling in response to glucose Carotenoids and fatty acids SRP067324, GSE92515 (Wang et al., 2019c; Zhang et al., 2019)
Coccomyxa sp.
(LA000219)
48.54 Response to arsenic treatment - Response to arsenic treatment Model organism and biofuels Koechler et al., 2016
Coccomyxa subellipsoidea
(C-169)
48.83 Response to CO2 supplementation; miRNA profiling - Response to nitric oxide, cadmium stress, carbon source, nitrogen starvation, phytohormones Biofuels GSE76638 PRJNA428141 (Kováčik et al., 2015; Allen et al., 2017; Liu et al., 2018a; Wang et al., 2019e)
Cyanidioschyzon merolae
(10D)
16.55 Response to diurnal cycle, different CO2 level, blue and red light, UV irradiance Response to low temperature acclimatization; photosystem II proteins Response to different CO2 level, diurnal cycle; hydrocarbon and lipid profiling in response to cyanobacterial Acyl-ACP Reductase overexpression Model organism GSE37673, GSE83828, GSE100372 (Krupnik et al., 2013; Rademacher et al., 2016; Nikolova et al., 2017; Miyagishima et al., 2019)
Dunaliella salina
(CCAP 19/18)
343.7 Response to osmotic and oxidative stress, nitrogen depletion, salinity, high light; different growth phases Response to arsenate, high salinity, high light and high bicarbonate ion level; flagella composition Response to nitrogen starvation Halophile, Biofuels, β-carotene and glycerol production Katz et al., 2007; Jia et al., 2009, 2016; Gu et al., 2014; Ge et al., 2016; Lv et al., 2016; Zhao et al., 2016; Wei et al., 2017b; Wang et al., 2019d
Emiliana huxleyi
(CCMP1516)
167.68 Response to nitrogen, sulfate and phosphorus starvation, calcium concentrations, elevated temperature and CO2 Response to different calcium concentration Response to host-virus (E. huxleyi virus) interaction, phosphorus and nitrogen starvation; lipidomic Coccolithophore GSE24341, E-MTAB-2274, SRP017794, SRX756940 (Benner et al., 2013; Rokitta et al., 2014; Hunter et al., 2015; McKew et al., 2015; Wördenweber et al., 2018)
Fistulifera solaris
(JPCC DA0580)
49.74 Response of nutrient depleted and replete conditions on lipid accumulation and its degradation Lipid droplet proteins - Biofuels DRA002404 (Nonoyama et al., 2019)
Fragilariopsis cylindrus
(CCMP1102)
80.54 Response to temperature, high CO2, prolonged darkness, and nitrogen and iron limitation; small RNA profiling Response to temperature, salinity stress, prolonged darkness, high CO2, iron starvation Response to different growth phases Psychrophile E-MTAB-5024, GSE57987 (Lyon et al., 2011; Boroujerdi et al., 2012; Kennedy et al., 2019)
Galdieria sulphuraria
(074W)
13.71 Response to cold acclimation Photosystem-II analysis - Extremophile PRJNA487158, GSE89169 (Thangaraj et al., 2010)
Guillardia theta
(CCMP2712)
87.15 Small RNA profiling under light and dark conditions, mRNA splicing analysis Response to different light intensities - Eukaryote endosymbiosis GSE124831, SRR747855 (Kieselbach et al., 2018)
Haematococcus pluvialis
(SAG 192.80)
365.78 Response to high light, salinity, iron, acetate, salicylic acid and jasmonic acid, nitrogen depletion and repletion, photooxidative stress; distinct growth phases Cell wall protein, astaxanthin accumulation, response to high light stress, salicylic acid, and jasmonic acid Lipid analysis, pigments and protein profiling, live single-cell analysis Carotenoids Wang et al., 2004; Tran et al., 2009; Peled et al., 2011; Gu et al., 2014; Recht et al., 2014; Su et al., 2014; Gao et al., 2016; Baumeister et al., 2019; Luo et al., 2019
Helicosporidium sp.
(ATCC 50920)
12.37 Transition from free-living organism to obligate intracellular parasite - - Parasite Pombert et al., 2014
Klebsormidium nitens
(NIES-2285)
104.21 Response to auxin treatment and cold stress - Response to cold stress Tolerance to UV and harsh conditions PRJDB4958, PRJNA500592 (Nagao et al., 2008)
Micromonas commoda (RCC299) 21.11 Response to different light regimes and ultra-violet light stress Response to chronic phosphate limitation and subsequent relief, high light and UV-radiation - Marine phytoplankton Cuvelier et al., 2017; Guo et al., 2018
Micromonas pusilla
(CCMP1545)
21.96 Response to phycodnavirus MpV-SP1 infection, phosphate deplete and replete, day-night cycle Phosphate deplete and replete condition, day-night cycle Response to phosphate deplete and replete condition; different growth phases, Marine phytoplankton PRJNA422663 (van Baren et al., 2016; Waltman et al., 2016; Kujawinski et al., 2017)
Micromonas sp.
(ASP10-01a)
19.58 Normal growth conditions - - Marine phytoplankton van Baren et al., 2016
Monoraphidium neglectum
(SAG 48.87)
69.71 Nitrogen deprivation - - Biofuels PRJNA221625 (Jaeger et al., 2017)
Nannochloropsis gaditana
(CCMP1894)
30.86 Response to light intensity regimes and nitrogen replete and deplete condition Fresh and atomized biomass Response to light intensity regimes and nitrogen deprivation / Genome scale metabolic model Biofuels Radakovits et al., 2012; Sorigue et al., 2016; Ajjawi et al., 2017; Shah et al., 2017; Fernandez-Acero et al., 2019; Patelou et al., 2020
Nannochloropsis limnetica
(CCMP505)
33.51 - - Nitrogen deprivation Biofuels Sorigue et al., 2016
Nannochloropsis oceanica
(LAMB2011)
29.26 Response to different CO2 levels, phosphorus and nitrogen limitation, light and dark cycle, fresh water acclimation; transition from quiescence to autotrophy Response to long-term nitrogen starvation, low CO2; single-cell-level phenotypic heterogeneity Response to osmotic downshift and nitrogen depletion Biofuels Dong et al., 2013; Pal et al., 2013; Sorigue et al., 2016; Poliner et al., 2018; Chen et al., 2019; Wei et al., 2019
Nannochloropsis oculate
(CCMP525)
26.27 - Nitrogen deprivation, cadmium stress Nitrogen deprivation Lipids and protein content Kim et al., 2005; Sorigue et al., 2016; Tran et al., 2016
Ostreococcus lucimarinus
(CCE9901)
13.2 - - Genome scale metabolic model Small genome Krumholz et al., 2012
Ostreococcus tauri
(RCC4221)
13.03 Response to OtV5 virus infection, light and dark cycle, iron limitation, and high light; life cycle stages Phosphoproteome in response to casein kinase 2, light dark cycle Glycerolipid profiling under nutrient deprived condition, diurnal variations, nitrogen deprivation / Genome Scale metabolic model Small genome Krumholz et al., 2012; Martin et al., 2012; Hindle et al., 2014; Le Bihan et al., 2015; Lelandais et al., 2016; Sorigue et al., 2016; Degraeve-Guilbault et al., 2017; Hirth et al., 2017
Parachlorella kessleri
(NIES-2152)
59.18 Response to salt stress and sulfur deplete and replete Salt stress Nitrogen, sulfur and phosphorus deprivation Ota et al., 2016a,b; Shaikh et al., 2019; You et al., 2019
Phaeodactylum tricornutum
(CCAP 1055/1)
27.45 Response to nitrogen, iron, carbon and phosphorus deprivation, cadmium stress, mixotrophic growth, grazing stress, different light intensities, and regimes, salicylic acid; non-coding microRNA Response to nitrogen limitation, oxidative and dark stress; phosphoproteomics under high light, nitrogen, and iron deficiency Response to blue and red light, nitrogen and phosphorus deprivation; glycerolipid profile; mixotrophic growth / Genome scale and core metabolic model Model organism PRJEB11970, SRX648639 (Chen et al., 2014; Ge et al., 2014; Jungandreas et al., 2014; Rosenwasser et al., 2014; Yang et al., 2014; Abida et al., 2015; Alipanah et al., 2015; Feng et al., 2015; Bai et al., 2016; Longworth et al., 2016; Sorigue et al., 2016; Yoneda et al., 2016; Villanova et al., 2017; Remmers et al., 2018; Smith et al., 2019)
Picochlorum sp.
(SENEW3 / DOE 101)
13.39 / 15.25 Response to salinity stress and high temperature - - Biofuels PRJNA245752, PRJNA389600
Scenedesmus sp.
(ARA3, ARA)
93.24 Response to phosphorus and nitrogen starvation, lipid accumulation Response to salinity stress; lipid accumulation Response to salinity and arsenic stress; lipid accumulation Biofuels PRJNA428298 (Chu et al., 2011; Arora et al., 2018, 2019; Wang et al., 2019b)
Scenedesmus obliquus
(UTEX393)
107.72 Response to diurnal changes and nC60; wild type and starch less mutant comparison Thylakoid membrane proteome, toxicity of silver nanoclusters Response to nC60 and silver nanoparticles; different photoperiod and growth phases Lipid and biomass E-MTAB-7009 (Kantzilakis et al., 2007; Du et al., 2017; Zhang et al., 2017; Vendruscolo et al., 2019; Wang et al., 2019a)
Symbiodinium minutum
(Mf 1.05b.01)
609.48 Diurnal cycle, cultured, and freshly isolated cells - Response to acidification Coral symbiont PRJNA544863 (Jiang and Lu, 2019)
Symbiodinium microadriaticum
(CCMP2467)
808.2 Response to different temperature, dark, and salinity stress; normal growth conditions, miRNA profiling - Response to environmental variation Coral symbiont GSE47373, GSE47372 (Klueter et al., 2015; Aranda et al., 2016)
Tetraselmis striata
(LANL1001)
227.95 Normal growth - - PRJNA231566, https://www.imicrobe.us/#/projects/104
Thalassiosira oceanica
(CCMP1005)
92.18 Response to iron and copper Response to iron and copper; extracellular superoxide production - Model organism PRJNA382002, SRA045825 (Lommer et al., 2012; Diaz et al., 2019)
Thalassiosira pseudonana
(CCMP1335)
32.44 Response to nitrogen and phosphorus deprivation, salinity, light intensity, triphenyltin chloride, silicon, CO2 levels, source of light, and nitrogen Response to nitrogen and phosphorus starvation, light intensity, salinity, triphenyltin chloride, CO2 levels, silicon, micronutrients deficiency, benzo(a)pyrene, K. brevis allelopathy; composition of nano- and micropatterned biosilica cell wall, mitochondrial and plastid proteome Response to phosphate deplete and replete condition, cobalamin scarcity; K. brevis allelopathy Model organism Carvalho and Lettieri, 2011; Dyhrman et al., 2012; Du et al., 2014; Kettles et al., 2014; Kustka et al., 2014; Luo et al., 2014; Poulson-Ellestad et al., 2014; Yi et al., 2014; Jian et al., 2017; Kujawinski et al., 2017; Chen et al., 2018; Heal et al., 2019; Schober et al., 2019
Trebouxia gelatinosa
(LA000220)
61.73 Response to dehydration and subsequent rehydration - - Colonization through symbiosis PRJNA213702
Volvox carteri f. magariensis
(Eve)
137.68 Response to low dose of UV-B radiation; somatic and reproductive cells - - Multicellular alga, model organism E-MTAB-5691 and GSE104835
Yamagishiella unicocca
(NIES-3982)
134.24 Normal growth condition - - Multicellular alga, model organism PRJNA532307