Table 1.
Number of hg38 genome matches, considering 0–3 mismatches, using two different methods (A, Aho–Corasick; B, Bowtie).
| Target | CRISPR site | No. of mismatches | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Spacer range | Spacer sequence | PAM | 0 | 1 | 2 | 3 | ||||
| A0 | B0 | A1 | B1 | A2 | B2 | A3 | B3 | ||||
| CFTR | chr7:117559575-94:+ | ATTAAAGAAAATATCATCTT | TGG | 1 | 1 | 7 | 7 | 145 | 143 | 2,304 | 2,277 |
| chr7:117559606-25:− | TCTGTATCTATATTCATCAT | AGG | 1 | 1 | 7 | 7 | 125 | 124 | 1701 | 1,672 | |
| HBB | chr11:5227003-22:− | CATGGTGCATCTGACTCCTG | AGG | 2 | 2 | 0 | 0 | 14 | 13 | 210 | 208 |
| chr11:5226984-7003:+ | GTAACGGCAGACTTCTCCTC | AGG | 1 | 1 | 0 | 0 | 7 | 7 | 83 | 82 | |
| HEXA | chr15:72346551-70:+ | TGTAGAAATCCTTCCAGTCA | GGG | 1 | 1 | 0 | 0 | 25 | 25 | 295 | 292 |
| chr15:72346558-77:+ | ATCCTTCCAGTCAGGGCCAT | AGG | 1 | 1 | 0 | 0 | 6 | 6 | 201 | 199 | |
| PRNP | chr20:4699568-87:+ | AGCAGCTGGGGCAGTGGTGG | GGG | 1 | 1 | 2 | 2 | 95 | 88 | 904 | 891 |
| chr20:4699569-88:+ | GCAGCTGGGGCAGTGGTGGG | GGG | 1 | 1 | 12 | 12 | 98 | 93 | 1,043 | 1,024 | |
| chr20:4699575-94:+ | GGGGCAGTGGTGGGGGGCCT | TGG | 1 | 1 | 2 | 2 | 55 | 54 | 857 | 826 | |
| chr20:4699578-97:+ | GCAGTGGTGGGGGGCCTTGG | CGG | 1 | 1 | 0 | 0 | 32 | 31 | 417 | 412 | |