Skip to main content
. 2020 Sep 9;3(11):e202000674. doi: 10.26508/lsa.202000674

Figure S5. Effect of siRNA-mediated Cdx2 knockdown on cell cycle repressors in trophoblast stem (TS) cells.

Figure S5.

(A) Quantitative real-time PCR analysis for Cdx2 in TS cells and differentiated trophoblast cells. (B) Western blot analysis for CDX2 in TS cells and differentiated trophoblast cells. Densitometric analysis of the Western blot is shown in the right. (C) Real-time PCR analysis of Cdx2 in TS cells using increasing concentrations of two Cdx2 siRNAs mix in equal amounts. (D) Quantitative real-time PCR analyses for cell cycle repressors in scrambled and si-cdx2 transfected TS cells. (E) Immunoblot analyses of CDX2 and cell cycle repressors in scrambled and si-cdx2 transfected TS cells. (F, G) Densitometric analysis of Western blots for CDX2 (F) and cell cycle repressors (G) in scrambled and si-cdx2 transfected TSCs. Data are presented in mean ± SEM of three replicates (n = 3). *P < 0.05; **P < 0.005; ***P < 0.0005.