Figure S6. Effect of CDX2 overexpression on cell cycle repressors in trophoblast stem (TS) cells.
(A) Quantitative real-time PCR analysis of Cdx2 in TS cells transfected with either empty FLAG vector or FLAG-CDX2. (B) Quantitative real-time PCR analyses of cell cycle repressors in TS cells transfected with either empty FLAG vector or FLAG-CDX2. (C) Immunoblot analyses of CDX2 and cell cycle repressors in control (FLAG) and CDX2 overexpressed (CDX-OE) TS cells. (D, E) Densitometric analysis of Western blots for CDX2 (D) and cell cycle repressors (E). Data are presented in mean ± SEM of three replicates (n = 3). *P < 0.05; **P < 0.005; ***P < 0.0005.