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. 2020 Sep 16;5(5):e00728-20. doi: 10.1128/mSphere.00728-20

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Copyright © 2020 Boekhoud et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Overexpression of σ^B is toxic for C. difficile and leads to plasmid loss. (A) Tenfold serial dilutions on brain heart infusion broth supplemented with 0.5% (wt/vol) yeast-extract (BHIY) agar plates of the 630Δerm (wild-type), AP34 (P_tet-sluc^opt), and JC096 (P_tet-sigB) strains. Similar results were obtained for strains IB58 and IB61 (data not shown). (B) Percentages of cells retaining the plasmid in AP34 (P_tet-sluc^opt) and JC096 (P_tet-sigB). (C) Percentages of cells retaining the plasmid in strains IB61 (sigB::CT P_tet-sluc^opt) and IB58 (sigB::CT P_tet-sigB). Percentages were calculated based on the ratio of CFU/ml of the paired selective (with thiamphenicol) and nonselective (without thiamphenicol) plates. *, P < 0.05; ****, P ≤ 0.0001, as determined by an unpaired Student’s t test (n = 3).