TABLE 1.
Setup of the DNA array and numbers of differentially expressed genes, including numbers of positively and negatively regulated genes
| Hybridization setup | Control | Target | Conditions | No. of genesa |
||
|---|---|---|---|---|---|---|
| DE | POS | NEG | ||||
| 1 | sigB::CT Ptet-slucopt (IB61) | sigB::CT Ptet-sigB (IB58) | Lincomycin (20 μg/ml), thiamphenicol (20 μg/ml), no ATc | 5 | 4 | 1 |
| 2 | sigB::CT Ptet-slucopt (IB61) | sigB::CT Ptet-sigB (IB58) | Lincomycin (20 μg/ml), thiamphenicol (20 μg/ml), ATc (100 ng/ml) | 183 (178) | 167 (163) | 16 (15) |
| 3 | sigB::CT Ptet-slucopt (IB61) | sigB::CT Ptet-sigB (IB58) | Lincomycin (20 μg/ml), no thiamphenicol, no ATc | 5 | 4 | 1 |
| 4 | sigB::CT Ptet-slucopt (IB61) | sigB::CT Ptet-sigB (IB58) | Lincomycin (20 μg/ml), no thiamphenicol, ATc (100 ng/ml) | 150 (145) | 136 (132) | 14 (13) |
a
Numbers in brackets correspond to the number of differentially expressed genes after subtracting the differentially expressed genes identified in hybridizations 1 and 3 (that are not dependent on sigB induction). DE, differentially expressed; POS, positively regulated; NEG, negatively regulated.