TABLE 3.
Oligonucleotides used in this study
| Name | Sequence (5′–3′)a | Description | Source or reference |
|---|---|---|---|
| Cdi-sigB-F | GTAGCTAATGCTACACATTAC | Verification of sigB ClosTron mutant | This study |
| Cdi-sigB-R | CAGTCATCTGTGATATCCCTAG | Verification of sigB ClosTron mutant | This study |
| EBSuniversal | CGAAATTAGAAACTTGCGTTCAGTAAA | Verification of sigB ClosTron mutant | 49 |
| ErmRAM-F | ACGCGTTATATTGATAAAAATAATAGTGGG | Verification of sigB ClosTron mutant | 49 |
| ErmRAM-R | ACGCGTGCGACTCATAGAATTATTTCCTCCCG | Verification of sigB ClosTron mutant | 49 |
| oIB-1 | TAGCCATATGAAAAATGTAGCTAATGCTACAC | Forward primer for sigB cloning in pET21b, contains a NdeI restriction site | This study |
| oIB-2 | ACTGCTCGAGTAAATTTTTTTCATATTCTTTTTTCAG | Reverse primer for sigB cloning in pET21b, contains an XhoI restriction site | This study |
| oIB-14 | GTACGGTACCTTTACATATACTATATATGTTAGAAAAAC | Forward primer for Pcd0872 containing a KpnI restriction site | This study |
| oIB-15 | GGTAGAGCTCATAGTTTACTCCTTTTTGTTATAATTG | Reverse primer for Pcd0872 containing a SacI restriction site | This study |
| oIB-26 | GGAAGGTACCGTTGAATAAAGTATTTATTTTCCATG | Forward primer for Pcd3412 containing a KpnI restriction site | 20 |
| oIB-27 | GGTAGAGCTCAGTATCACTCCTTTTTTCGAAC | Reverse primer for Pcd3412 containing a SacI restriction site | 20 |
| oIB-80 | ctagcataaaaataagaagcctgcatttgcAAATTTACGAAAAGCTTGC | Forward primer for Pcd0350 | 20 |
| oIB-82 | ctagcataaaaataagaagcctgcatttgcTTGTGTTTAAGGGATTTTGAAAG | Forward primer for Pcd2963 | 20 |
| oIB-90 | ctagcataaaaataagaagcctgcatttgcATGTAAAGAAGCCGAAGAAG | Forward primer for Pcd3605 | This study |
| oIB-92 | ctagcataaaaataagaagcctgcatttgcGAATAAAAAAGGTGGTGTC | Forward primer for Pcd3614 | 20 |
| oIB-94 | agctattaataattttttacttggtctcatTTTTACCTCCATGTAACATTTATTG | Reverse primer for Pcd0350 | 20 |
| oIB-95 | agctattaataattttttacttggtctcatAATTAAATCCTTCCTTACATTGTAATTAC | Reverse primer for Pcd2963 | 20 |
| oIB-99 | agctattaataattttttacttggtctcatATTTCAGCCCTCCATATTTG | Reverse primer for Pcd3605 | This study |
| oIB-100 | agctattaataattttttacttggtctcatATAAACACCCTCCTATTCTTTG | Reverse primer for Pcd3614 | 20 |
| oWKS-1498 | GAGCTCCTGCAGTAAAGGAGAAAATTTTATGAAAAATGTAGCTAATGCTACAC | Forward primer for sigB CDS | This study |
| oWKS-1499 | GGATCCTTATAAATTTTTTTCATATTCTTTTTTCAG | Reverse primer for sigB CDS | This study |
| oWKS-1513 | GAGCTCAAATTTGAATTTTTTAGGGGGAAAATACCATGCATCATCACCATCACCACGGTTCCGAAATCGGTACTGGCTTTCC | Oligonucleotide used for end labeling | This study |
| oWKS-1506 | GAGCTCAAATTTGAATTTTTTAGGGGGAAAATACCATGGTTTCAAAAGGAGAAGAATTATTTAC | Oligonucleotide used for end labeling | This study |
a
Restriction sites are underlined, and 30-bp overlapping regions used in Gibson Assembly are indicated in lowercase letters.