A. fumigatus SlnA and ShoA branches are genetically interacting. (A) Protein organization of A. fumigatus SlnA. (B to O) Growth phenotypes of the wild-type strain and the ΔslnA, ΔshoA, ΔmsbA, ΔopyA, ΔslnA ΔshoA, ΔslnA ΔmsbA, and ΔslnA ΔopyA mutants were determined. The strains were grown for 4 days at 37°C (B) on MM and YAG (C), MM plus Congo red (CR) (D and J), MM plus calcofluor white (CFW) (E and K), MM plus CaCl2 (F and L), MM plus sorbitol (G and M), MM plus menadione (H and N), and MM plus caspofungin (I and O), and their radial growth was quantified. The results shown represent the means of measurements of the diameter of 3 colonies for each strain ± standard deviation. The data were analyzed (Prism, GraphPad) using two-way ANOVA followed by Bonferroni posttests. The levels of significance compared to the wild-type results are indicated as follows: *, P < 0.1; **, P < 0.01; ***, P < 0.001.