FIG 2.

A. fumigatus ShoA, MsbA, and OpyA. (A to C) Protein organization of A. fumigatus ShoA (A), MsbA (B), and OpyA (C). (D to J) Growth phenotypes of the wild-type strain and the ΔshoA, ΔmsbA, ΔopyA, ΔshoA::shoA⁺, ΔmsbA::msbA⁺, and ΔopyA::opyA⁺ mutants. The strains were grown for 4 days at 37°C (D) on MM or YAG (E), MM plus Congo red (CR) (F), MM plus calcofluor white (CFW) (G), MM plus CaCl₂ (H), MM plus sorbitol (I), MM plus menadione (J), and MM plus caspofungin (K), and their radial growth was quantified. The results shown represent the means of measurements of the diameter of 3 colonies for each strain ± standard deviation. The data were analyzed (Prism, GraphPad) using two-way ANOVA followed by Bonferroni posttests. The levels of significance compared to the wild-type results are indicated as follows: *, P < 0.1; **, P < 0.01; ***, P < 0.001.