FIG 4.

A. fumigatus ShoA, MsbA, and OpyA are important for SakA and MpkA phosphorylation. Western blotting assays of SakA and MpkA phosphorylation in the wild-type strain and single mutants (A and C) or in the wild-type strain and double mutants (B and D) in response to 1.2 M sorbitol for different periods of time (A and B) and in response to caspofungin 0.25, 2.0, or 8.0 μg/ml for 1 h (C and D) were performed. Anti-P-p38 SakA and anti-44/42 MpkA antibodies were used to detect the phosphorylation of SakA and MpkA, respectively, while anti-γ-tubulin was used to detect γ-tubulin. Signal intensities were quantified using ImageJ software, and ratios of (P)-SakA to γ-tubulin or (P)-MpkA to γ-tubulin were calculated.