FIG 5.

A. fumigatus shoA overexpression inhibits growth. (A) The wild-type and xylp::shoA strains were grown in MM plus 1% glucose for 16 h at 37°C and transferred to MM plus 1% xylose for 30 to 120 min at 37°C. The results shown represent the means of measurements of the diameter of 3 colonies for each strain ± standard deviation. Gene expression was normalized using tubA (Afu1g10910). Standard deviations present averages of results from three independent biological repetitions (each performed with 2 technical repetitions). (B) The wild-type, xylp::shoA, and ΔshoA strains were grown either on MM plus 1% glucose or on MM plus 1% xylose for 5 days at 37°C. (C) The wild-type, xylp::shoA, and ΔshoA strains were grown in MM plus 1% glucose for 16 h at 37°C and transferred to MM plus 1% xylose for 30 to 120 min at 37°C. Western blotting assays of SakA and MpkA phosphorylation were performed. Anti-P-p38 SakA and anti-44/42 MpkA antibodies were used to detect the phosphorylation of SakA and MpkA, respectively.