The ΔshoA, ΔmsbA, and ΔopyA single and double null mutants showed reduced growth on glucose 1% (A), acetate 1% (B), glycerol 1% (C), or ethanol 1% (D) as a single carbon source. The wild-type and null mutant strains were grown for 5 days at 37°C. The results shown represent the means of measurements of the diameter of 3 colonies for each strain ± standard deviation. The data were analyzed (Prism, GraphPad) using two-way ANOVA followed by Bonferroni posttests. The levels of significance compared to the wild-type results are indicated as follows: *, P < 0.1; **, P < 0.01; ***, P < 0.001.