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. 2020 Sep 3;11:2008. doi: 10.3389/fimmu.2020.02008

Table 2.

Best HLA-II candidate epitopes for South America in SARS-CoV-2 proteins.

Best HLA-II candidate epitopes for South America N° of alleles with WAF ≥ 0.05 covered by country Coverage score References
Protein Start End Peptide Region Experimental IEDB ID ARG BOL BRA CHI COL ECU PAR PER VEN
S 1013 1027 IRAAEIRASANLAAT CH LTM 100428 5 5 5 4 5 7 4 7 4 8.148 New
1014 1028 RAAEIRASANLAATK 100428 6 5 7 4 5 7 5 7 5 9.000
1152 1166 LDKYFKNHTSPDVDL HR2 ML 35205 4 4 4 3 4 5 5 6 3 6.760 Ahmed et al. (66)
1153 1167 DKYFKNHTSPDVDLG 9006 4 4 4 3 4 5 5 6 3 6.760
S 61 75 NVTWFHAIHVSGTNG NTD - - 4 4 4 3 4 5 4 4 4 6.474 New
114 128 TQSLLIVNNEATNVVI 4 4 5 3 4 6 5 6 3 7.045
115 129 QSLLIVNNATNVVIK 5 4 6 4 5 5 5 5 4 7.719
116 130 SLLIVNNATNVVIKV 4 3 5 3 4 4 5 4 4 6.474
206 220 KHTPINLVRDLPQGF 4 3 4 3 4 5 3 5 3 6.017
207 221 HTPINLVRDLPQGFS 5 4 5 3 4 6 5 6 4 7.412
208 222 TPINLVRDLPQGFSA 4 4 3 3 4 5 2 6 3 6.017
216 230 LPQGFSALEPLVDLP 3 4 3 3 3 5 5 6 4 6.450
217 231 PQGFSALEPLVDLPI 3 4 3 3 3 5 5 6 4 6.450
308 322 VEKGIYQTSNFRVQP RBD - - 4 5 5 3 4 7 5 7 3 7.531
309 323 EKGIYQTSNFRVQPT 5 5 6 4 5 7 5 7 4 8.490
313 327 YQTSNFRVQPTESIV 3 5 3 3 3 6 5 6 3 6.593
314 328 QTSNFRVQPTESIVR 6 5 7 4 5 6 5 6 5 8.714
315 329 TSNFRVQPTESIVRF 6 5 7 4 5 7 5 7 5 9.000
316 330 SNFRVQPTESIVRFP 3 4 5 3 3 5 5 5 4 6.593
430 444 TGCVIAWNSNNLDSK 4 4 6 3 4 6 5 6 4 7.388
689 703 SQSIIAYTMSLGAEN - - - 3 5 3 3 4 7 4 7 3 6.879
690 704 QSIIAYTMSLGAENS 4 5 4 3 4 7 5 7 3 7.388
785 799 VKQIYKTPPIKDFGG 3 5 2 3 3 5 4 6 3 6.107
801 815 NFSQILPDPSKPSKR FP - - 5 4 5 3 4 6 4 6 4 7.212
802 816 FSQILPDPSKPSKRS 5 4 5 3 4 6 5 6 4 7.412
1059 1073 GVVFLHVTYVPAQEK BH - - 3 5 3 3 3 7 5 7 4 7.079
1060 1074 VVFLHVTYVPAQEKN 3 4 2 3 3 5 4 6 4 6.107
1098 1112 NGTHWFVTQRNFYEP SD3 - - 4 4 4 3 3 5 5 5 4 6.617
1099 1113 GTHWFVTQRNFYEPQ 4 4 4 3 3 6 5 6 4 6.902
1110 1124 YEPQIITTDNTFVSG 4 4 4 3 4 5 5 5 4 6.817
1111 1125 EPQIITTDNTFVSGN 4 4 6 3 4 6 5 6 4 7.388
1126 1140 CDVVIGIVNNTVYDP - - - 4 3 5 3 4 4 5 4 4 6.474
M 7 21 TITVEELKKLLEQWN Virion Surface - - 2 2 2 1 1 3 3 3 2 3.326 New
E 1 15 MYSFVSEETGTLIVN Virion Surface - - 0 2 1 1 1 2 0 2 1 1.764 New

These candidate epitopes were selected from our prediction based on their scores (rank for NetMHC and IC50 for MHCflurry), Coverage Score (CS), and exposure (for Class-II only). Experimental evidence including the IEDB ID and experiment type (LTM: linearT_MHC, ML: MHC_ligand) is provided when available. Scientific articles already proposing these candidates are mentioned. Underlined residues indicate sites with predicted N-linked glycosylations. Residues in bold represent positive selection pressure sites.