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. 2020 Sep 16;11(9):764. doi: 10.1038/s41419-020-02930-y

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Western blot analysis of BCAT1 expression in hypoxic PASMCs (n = 8). b Subcellular distribution of BCAT1 in PASMCs determined by immunofluorescence analysis. Scale bars: 50 μm (n = 3). c The cellular expression of BCAT1 in the smooth muscle layer of lung tissues from hypoxic model rats determined by immunofluorescence staining analysis. Scale bar = 100 μm (n = 3). d BCAT1 protein levels in pulmonary arterial tissues of hypoxia model rats (n = 8). e Time course of BCAT1 expression of PASMCs at 0, 6, 12, 24, 48, and 72 h after hypoxia treatment (n = 6). Nor normoxia, Hyp hypoxia, Mct monocrotaline. Statistical analysis was performed with one-way ANOVA or the Student’s t test. All values are presented as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant.