Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 16;10:15169. doi: 10.1038/s41598-020-71669-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

LAMP3 inhibits cell growth and induces caspase-dependent apoptosis. (A) HSG and (B) A253 cells were transfected with 1.5 μg pME18S-empty or pME18S-LAMP3 plasmid, and then 2 × 10⁵ cells of each cells were re-plated 24 h post-transfection. The number of cells was counted 96 h after re-plating with Countess Automated Cell Counter (N = 3 independent experiment and 2 technical replicates of each experiment). (C,D) HSG cells and (E,F) A253 cells were transfected with different concentrations of pME18S-LAMP3 plasmid or a pME18S-empty plasmid as control. Seventy-two hours post-transfection, apoptotic cells were counted by using flow cytometry with APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (G,H) Number of apoptotic cells in control and LAMP3-overexpressing (OE) HSG cell cultures 24, 48 and 72 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 3). (I,J) Number of apoptotic cells in GFP⁺ control, LAMP3-OE and LAMP1-OE HSG cell cultures 48 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (K,L) Number of apoptotic cells in GFP⁺ control, LAMP3-OE and LAMP1-OE A253 cell cultures 48 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (M,N) Control and LAMP3-OE HSG cells were incubated with or without 20 μM Z-VAD-FMK (Z-VAD) for 20 h. Extent of apoptosis was determined by flow cytometry using APC Annexin V/7-AAD. Difference in rate of Annexin V⁺ cells in LAMP3-OE cell culture treated with or without 20 μM Z-VAD from that in control cell culture is shown (N = 4). *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t-test. Values shown are mean ± SD.

LAMP3 inhibits cell growth and induces caspase-dependent apoptosis. (A) HSG and (B) A253 cells were transfected with 1.5 μg pME18S-empty or pME18S-LAMP3 plasmid, and then 2 × 10⁵ cells of each cells were re-plated 24 h post-transfection. The number of cells was counted 96 h after re-plating with Countess Automated Cell Counter (N = 3 independent experiment and 2 technical replicates of each experiment). (C,D) HSG cells and (E,F) A253 cells were transfected with different concentrations of pME18S-LAMP3 plasmid or a pME18S-empty plasmid as control. Seventy-two hours post-transfection, apoptotic cells were counted by using flow cytometry with APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (G,H) Number of apoptotic cells in control and LAMP3-overexpressing (OE) HSG cell cultures 24, 48 and 72 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 3). (I,J) Number of apoptotic cells in GFP⁺ control, LAMP3-OE and LAMP1-OE HSG cell cultures 48 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (K,L) Number of apoptotic cells in GFP⁺ control, LAMP3-OE and LAMP1-OE A253 cell cultures 48 h after transfection, assessed by flow cytometry using APC Annexin V/7-AAD. Difference from control cells is shown (N = 4). (M,N) Control and LAMP3-OE HSG cells were incubated with or without 20 μM Z-VAD-FMK (Z-VAD) for 20 h. Extent of apoptosis was determined by flow cytometry using APC Annexin V/7-AAD. Difference in rate of Annexin V⁺ cells in LAMP3-OE cell culture treated with or without 20 μM Z-VAD from that in control cell culture is shown (N = 4). *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t-test. Values shown are mean ± SD.