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. 2020 Sep 16;10:15169. doi: 10.1038/s41598-020-71669-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP3-induced autoantigens release was independent of apoptosis. (A) Protein concentration in mixture of extracellular vesicles (EVs) isolated from control and LAMP3-OE HSG cells treated with or without Z-VAD. Data are presented as relative change in expression compared with control cells. (B) Western blotting analysis of TRIM21/SSA, SSB and α-fodrin in EVs isolated from HSG control and LAMP3-OE cells treated with or withour Z-VAD. Protein levels of (C) TRIM21/SSA, (D) SSB and (E) α-fodrin were normalized to flotillin-1 level, and the value was plotted. Uncropped images are provided in Supplementary Figure S6. **p < 0.01, ***p < 0.001, unpaired Student’s t-test.