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. 2020 Sep 16;11(9):763. doi: 10.1038/s41419-020-02945-5

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© The Author(s) 2020

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Fig. 2 — BMDMs were treated with IL-4 or LPS to induce M2 or M1 polarization. a Quantification of F4/80⁺CD206⁺ ratio in IL-4-treated BMDMs, and F4/80⁺CD86⁺ ratio in LPS-treated BMDMs. b RT-qPCR of MM2P levels in BMDMs of each group. c The IL-4-treated BMDMs were transfected with sh-NC or sh-MM2P#1/2, whereas the LPS-treated BMDMs were transfected with pcDNA3.1 or pcDNA3.1/MM2P. RT-qPCR of MM2P levels in BMDMs of each group. d Quantification of F4/80⁺CD206⁺ ratio in IL-4-treated BMDMs, and F4/80⁺CD86⁺ ratio in LPS-treated BMDMs. e RT-qPCR of MM2P levels in BMDMs of each group. f Western blots of p-STAT1, STAT1, p-STAT6, STAT6, p-STAT3, STAT3, and quantification of p-STAT1/STAT1, p-STAT6/STAT6, and p-STAT3/STAT3 in BMDMs treated with IL-4 and LPS. **P < 0.01. The error bar expressed as mean ± SD of three independent experiments in triplicates.