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. 2020 Sep 16;11(9):763. doi: 10.1038/s41419-020-02945-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Pulldown assay followed by mass spectrometry identified the interaction between FUS and MM2P, which was confirmed by western blot. b, c RIP assay for the abundance of MM2P in FUS precipitates, and the abundance of SOX9 mRNA in the precipitates of FUS. d Pulldown assay for the abundance of SOX9 in the pulldown of the MM2P biotin group after silencing FUS. e RT-qPCR and western blot analysis of FUS mRNA and protein levels under MM2P depletion. f Levels of FUS and MM2P in IL-13/IL-4-induced M2 macrophages after silencing FUS. g Three potential FUS binding sites on SOX9 mRNA predicted by CLIP experimental data from Starbase3.0. h Pulldown-western blot assay for the enrichment of FUS in the pulldown of SOX9 mRNA. i RIP experiments confirmed the interaction between FUS and SOX9 mRNA at site 3 rather site 1 and 2. j SOX9 mRNA stability after Actinomycin D treatment, responding to FUS knockdown. k SOX9 mRNA and protein expressions under the silence of FUS in IL-13/IL-4-induced M2 macrophages. l mRNA stability of SOX9 in IL-13/IL-4-induced M2 macrophages, in response to MM2P knockdown. **P < 0.01. The error bar expressed as mean ± SD of three independent experiments in triplicates.