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. 2020 Sep 16;10:15209. doi: 10.1038/s41598-020-72214-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Incorporation of HMC3 human microglia into hBORGs. HMC3 microglia (1 × 10⁶ cells) were infected with HIV-1 (green) or mock-infected, membrane-labeled (red) and were added to hBORGs labeled with Hoechst-stained (blue). After 24 h, microglia incorporated-hBORGs were transferred to a new plate and maintained for an additional 15 days for further analyses. White arrowheads point to HIV-infected microglia (green) in insert a and b, and White asterisks in insert a and b point to uninfected microglia (red). Scale bar is 200 μm and 50 μm in inserts.