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. 2020 Sep 16;9:164. doi: 10.1038/s41377-020-00399-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative fluorescence images to observe the migratory capabilities of 5K-HA-HPPS, HPPS and 5K-HA-e to pLNs at 10min, 1, 3, 6 and 12 h after intradermal footpad injection. b Quantitative analysis of the mean fluorescence intensity of DiR-BOA in pLNs. c Fluorescence imaging was performed 12 h after injection on resected pLNs and sciatic LNs (sLNs). Scale bar: 2 mm. Data are presented as the mean ± SD (two-tailed t-test; n = 5 animals per group)