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. 2020 Sep 15;10:15090. doi: 10.1038/s41598-020-72004-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Viromers efficiently transfect murine macrophages. (a) Time-dependent appearance of GFP fluorescence in RAW264.7 macrophages observed by fluorescence microscopy after 6 h, 24 h and 30 h post transfection. Scale bar: 100 µm. (b,c) FACS analysis 24 h and 30 h after transfection of RAW264.7 macrophages using GFP-mRNA (100 ng/well) in comparison to untreated cells (UTC) and quantification (c) of GFP-positive cells in Q3 of the FACS plot. Mean ± SD, one-way ANOVA, Dunnett test, ***p < 0.001, n = 3. (d,e) Intracellular FLuc reporter gene expression (100 ng/well) analysed by FACS 24 h after transfection in comparison to UTC and isotype control (Iso) and quantification of FLuc-positive cells in Q3 of the FACS plot (e). Mean ± SD, one-way ANOVA, Dunnett test, *p < 0.05, n = 2. (f) Expression of 7ND (100 ng/well) in RAW264.7 macrophages analysed by ELISA 6 h, 24 h and 30 h post transfection. Graph shows box and whiskers (min to max), line at mean, two-way ANOVA, Sidak multiple comparison test, **p < 0.01, n = 2 (UTC), n = 6 (7ND). (g) Expression of sTNF-RII (100 ng/well) in RAW264.7 macrophages analysed by ELISA 6 h, 24 h and 30 h post transfection. Graph shows box and whiskers (min to max), line at mean, two-way ANOVA, Sidak multiple comparison test, ***p < 0.001, n = 2 (UTC), n = 6 (7ND).