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. 2020 Sep 16;10:15196. doi: 10.1038/s41598-020-71315-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Growth characteristics of HaCaT and HaSKpwC7 cells. (A) Growth curve of HaCaT and HaSKpwC7 cells. Cells were counted and normalized. Points represent means of triplicates of three independent experiments and the error bars are standard deviation. (B) Cell cycle distribution for both cell lines. While the HaSKpwC7 cells exhibit a near diploid genome size, HaCaT cells show a 1.4-fold increase in genome size, with no significant differences in the distribution of the cell cycle phases. (C) Images of S-phase positive cells identified by EdU pulse labelling (green), for HaCaT cells (top) and HaSKpwC7 cells (down). Quantification of S-phase positive cells in both cell lines, compiled for each cell line from three independent biological replicates (mean values and standard deviation). (D) Image of HaCaT cells (top) and HaSKpwC7 cells (down) stained with anti-H3S10p (red) to identify mitotic cells. Quantification of the mitotic fractions is based on cell size and mean H3S10p intensity, compiled from two biological replicates (mean and standard deviation). All assays were performed with HaSKpwC7 cells of passage 9–14 and HaCaT cells of passage 37–43.