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. 2020 Sep 15;10:15079. doi: 10.1038/s41598-020-71941-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative photomicrographs of right knee joint obtained from sham and OA rats at 10 and 20 weeks after OA induction stained with Safranin O/Fast green staining. Red color indicates articular cartilage. Green color indicates subchondral bone. Arrow indicates mild abrasion on the articular cartilage surface of tibial plateau. Arrow head indicates articular chondrocytes. Ma, marrow. Scale bars are 50 µm. (B) OARSI average grading score in the right knee joint obtained from sham and OA rats at 10 and 20 weeks after OA induction (n = 5 per group). *P < 0.05, **P < 0.01 vs. corresponding Sham group.